Digital microfluidics (DMF) is a lab-on-a-chip system platform integrated with a set of insulated electrodes for dispensing, moving, storing, mixing, reacting, or analyzing droplets. The device is based upon the manipulation of microdroplets to realize the experiment processing requirements. The new interdisciplinary research fields of digital microfluidics have big potential to revolutionize current technologies that require the handling of a small amount of fluid, a fast response, low costs, and automation.

Because of features of easier customizing and optimizing, clustered regularly interspaced short palindromic repeats (CRISPR) became more and more popular. It is a gene-editing tool that has changed researchers’ abilities to manipulate, detect and image DNA and RNA in various living cells. Recently RNA-guided endonuclease CRISPR-associated protein 9 (Cas9) receives much attention especially due to its basic research and prospects in treatment methods based on gene editing.

However, the CRISPR/Cas9 experiments are very complexed and time-consuming. DMF is a perfect tool for it. Our lab is using DMF device which is developed by Digi.Bio Company (Digi.Bio) to do yeast transformation using CRISPR/Cas9 in the heat shock method. And this project is part of the Early Access Program.

 

[Digital microfluidics designing software platform]