Publications

2022
2021
  • E. Walls, J. L. Martinez , E. A. del Rio Chanona  and L. Rios-Solis.  Definitive screening for accelerated Taxol biosynthetic pathway optimization and scale up in Saccharomyces cerevisiae cell factories. Manuscript accepted for publication to The Biotechnology Journal in September 2021.
  • P. Guaman-Bautista, E. Moreta U, C. G. O.-Arias, M. Torres-Arias, N. C. Kyriakidis, K. Malcı, N.Jonguitud-Borrego, L. Rios-Solis, E. Ramos-Martinez, A. López-Cortés, C. Barba-Ostria. Tracking SARS-CoV-2: novel trends and diagnostic strategies. Manuscript accepted for publication to the Journal of Diagnostics in September  2021.
  • Morris, R. Gacia Arrazola, L. Rios-Solis and P. A. Dalby. Biophysical characterization of the inactivation of E. coli transketolase by aqueous co-solvents. BioRxiv. .doi.org/10.1101/2020.06.09.1409882021. Manuscript submitted to Nature Scientific Report in September 2021
  • Malcı, E. Watts, J. Y. Auxillos, B. Nowrouzi,T. M. Roberts, H. Oss Boll, C. Zolnier Sousa do Nascimento, A. Andreou, S. Panke,  E. Wallace, A. Elfick, L. Rios-Solis. Standardisation of Synthetic Biology for Saccharomyces cerevisiae and emerging yeast species. Manuscript submitted to the Journal of ACS Synthetic Biology in August 2021.
  • Teworte, K. Malcı, L. E. Walls,  M. Halim, L. Rios-Solis. Recent advances in fed-batch microscale bioreactor design. Manuscript submitted to Biotechnology Advances in August 2021.
  • Jonguitud-Borrego, K. Malci ,M. Anand, E. Baluku, C. Webb, L. Liang, C. Barba Ostria, L. P. Guaman Bautista, L. Rios-Solis. Automated Screening for COVID-19 using an optimized one pot CRIPSR Cas9/ Replication Protein A assay. Manuscript submitted to the Journal of Molecular Diagnostics in July. 2021.
  • Temisak, J. Boonil, W. Yenchum, K. Hongthong, T. yat, L. Rios-Solis and P. Morris.  Accurate determination of meat mass fractions using DNA measurements for quantifying met adulteration. 2021. International Journal of Food Science and Technology. In Press.  BioRxiv. doi.org/10.1101/2020.06.14.150375.
  • Nowrouzi, L. Rios-Solis. The redox power: Hidden gem for improving whole-cell P450-catalysed terpenoid biosynthesis. 2021. Critical Reviews in Biotechnology. In Press.
  • Muhammad Fakhri Zainuddin, Chong Kar Fai, Arbakariya B Ariff, Leonardo Rios-Solis, Murni Halim. Current Pretreatment/Cell Disruption and Extraction Methods Used to Improve Intracellular Lipid Recovery from Oleaginous Yeasts. Microorganisms 9(2)
2020

L. Walls, K. Malci, B. Nowrouzi, R. Li, L. d’Espaux, J. Wong, J. Dennis, A. Semiao, S. Wallace, J. Martinez, J. D. Keasling, L. Rios-Solis. Optimising the biosynthesis of oxygenated and acetylated Taxol precursors in S. cerevisiae using advanced bioprocessing strategies. Biotech BioEng J. 2020. https://doi.org/10.1002/bit.27569

B. Nowrouzi, R. Li, L. E. Walls, L. d’Espaux, K. Malci, L. Liang, N. Jonguitud Borrego, A. I. Lerma Escalera, J. R. Morones-Ramirez, J. D. Keasling, L. Rios Solis. Enhanced production of taxadiene in S. cerevisiae. Manuscript accepted for publication at Microb. Cell Fac. 2020doi.org/10.1101/2020.06.08.13960

K. Malci, L E. Walls. L. Rios-Solis. Multiplex Genome Engineering Methods for Yeast Cell Factory Development. Epub ahead of publication. Front. Bioeng. Biotechnol. 2020. doi: 10.3389/fbioe.2020.589468

P. Morris, R. Garcia Arrazola, L. Rios-Solis, Paul A Dalby. Biophysical characterization of the inactivation of E. coli transketolase by aqueous co-solvents. BioRxiv. 2020. doi.org/10.1101/2020.06.09.140988

P. Thangsunan, S. Temisak, P. Morris, L. Rios-Solis, N. Suree. Combination of loop-mediated isothermal amplification and AuNP-oligoprobe colourimetric assay for pork authentication in processed-meat products. Paper accepted for publication at J. Food Anal. Methods. 2020doi.org/10.1101/2020.07.12.199091

S. Temisak, P. Thangsunan, J. Boonil, W. Yenchum, K. Hongthong, T. Yata, L. Rios-Solis, P. Morris. Accurate determination of meat mass fractions using DNA measurements for quantifying meat adulteration. BioRxiv 2020. doi.org/10.1101/2020.06.14.150375

L. E. Walls, L. Rios-Solis. Sustainable microbial isoprenoid derived advanced biojet fuel production: a review. J. Biotech. Bioeng. Epub ahead of publication. 2020doi:10.3389/fbioe.2020.599560

J. Beal, A. Go.i‐Moreno, C. Myers, A. Hecht, M. del Carmen de Vicente, M. Parco, M. Schmidt, K. Timmis, G. Baldwin, S. Friedrichs, P. Freemont, D. Kiga, E. Ordozgoiti, M. Rennig, L. Rios Solis, K. Tanner, V. de Lorenzo, M. Porcar. The long journey towards standards for engineering biosystems. EMBO Rep. 2020. 6; 21(5). doi.org/10.15252/embr.202050521

2018

P. Wilk, H. Murni, L. Rios-Solis. 2018. Recent Advances and Impacts of Microtiter Plate-Based Fermentations in Synthetic Biology and Bioprocess Development. Fermentation Microbiology and Biotechnology, 4th edition. Taylor and Francis Publishers. 18:359-372

 N.F Fazilah, A.B Ariff, M.E Khayat, L. Rios-Solis, M Halim. 2018. Influence of probiotics, prebiotics, synbiotics and bioactive phytochemicals on the formulation of functional yogurt. J. Funct. Foods 48, 387-399. doi.org/10.1016/j.jff.2018.07.039

 M. Othman, A. B. Ariff, M. R. Kapri, L. Rios-Solis, M. Halim. 2018. Growth enhancement of probiotic Pediococcus acidilactici by extractive fermentation of lactic acid exploiting anion-exchange resin. Fronts. Microbiol. 9, 2554. doi.org/10.3389/fmicb.2018.02554

J. Wong, T. de Rond, L. d’Espaux, C. V. Horst, I. Dev, L. Rios-Solis, J. Kirby, H. Scheller, Jay Keasling. 2018. High-titer production of lathyrane diterpenoids from sugar by engineered Saccharomyces cerevisiae. Metab. Eng. 45:142-148. doi.org/10.1016/j.ymben.2017.12.007

2017

M. Othman, A. B. Ariff, L. Rios-Solis, M. Halim. 2017. Extractive Fermentation of Lactic Acid in Lactic Acid Bacteria Cultivation: A Review. Fronts. Microbiol. 8: 2285 doi.org/10.3389/fmicb.2017.02285

J. Wong, L. Rios-Solis, J. D. Keasling. Microbial production of isoprenoids. 2017. Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Production of Fuels and Chemicals. Part of the series Handbook of Hydrocarbon and Lipid Microbiology Springer, Berlin, Germany pp. 21-24.

K. Markošov., I. Dolejš, R. Stloukal, L. Rios-Solis, M. Rosenberg, M. Micheletti, G. J. Lye, N. J. Turner, M. Rebroš. 2016. Immobilisation and kinetics of monoamine oxidase (MAO-N-D5) enzyme in polyvinyl alcohol gels. J. Mol. Cat. B. Enz. 129:69-74. doi.org/10.1016/j.molcatb.2016.04.009

2016

P. Morris, L. Rios-Solis, R. Garc.a-Arrazola, G. J. Lye and P. A. Dalby. 2016. Impact of cofactorbinding loop mutations on thermotolerance and activity of E. coli transketolase. Enz Microb Tech. 89:85-91. DOI: 10.1016/j.enzmictec.2016.04.003

L. Rios-Solis, P. Morris, H. Al-Bahrani, C. Du. Immobilized biospheres filtration system using unidirectional flow. U.K. Patent Application GB1602673.4, filed 16 February 2016. Patent Pending.

2015

L. Rios-Solis, B. Mothia, S. Yi, Z.Yuhong, M. Micheletti and G. J. Lye. 2015. High throughout monoamine oxidase (MAO-N) screening and kinetic model generation. J. Mol. Cat. B. Enz. 120-100-110. doi.org/10.1016/j.molcatb.2015.07.001

L. Rios-Solis, P. Morris, A. Odeleye, C. Grant, J.M. Ward, P.A. Dalby, F. Baganz and G.J. Lye. 2015. Modelling and optimization of the one-pot synthesis of chiral amino-alcohols based on microscale kinetic parameter determination. Chem. Eng Sci. 122: 360–372. DOI:10.1016/J.CES.2014.09.046

2014

C.J. Du, L. Rios-Solis, J.M. Ward, P. A. Dalby, G. J. Lye. 2014. Evaluation of the CV2025 ω-transaminase for the bioconversion of lignin breakdown products into value-added chemicals. Biocat. Biotrans. 32:302-313. doi.org/10.3109/10242422.2014.976632

M. Halim, L. Rios-Solis. M. Micheletti, J. M. Ward and G.J. Lye. 2014. Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ω-transaminase synthesis of chiral amines. Bioprocess Biosyst. Eng. 37:931-41. DOI: 10.1007/s00449-013-1065-5

2013

L. Rios-Solis, N. Bayir, M. Halim., J. Hao, F. Baganz., G.J. Lye. 2013. Non-linear kinetic modelling of reversible bioconversions: application to the transaminase catalysed synthesis of chiral amino-alcohols. Biochem. Eng. J. 73:38-48. doi.org/10.1016/j.bej.2013.01.010

2011

L. Rios-Solis, M. Halim, A. C.zares, P. Morris, J. M. Ward, H.C. Hailes, P.A. Dalby, F. Baganz and G.J. Lye. 2011. A toolbox approach for the rapid evaluation of multi-step enzymatic syntheses comprising a ‘mix and match’ E. coli expression system with microscale experimentation. Biocat. Biotrans. 29: 192-203. doi.org/10.3109/10242422.2011.609589

2010

A. C.zares, J. L. Galman, L. G. Crago, M. E. B. Smith, J. Strafford, L. Rios-Solis, G. J. Lye, P. A. Dalby and H. C. Hailes. 2010. Non-alpha-hydroxylated aldehydes with evolved transketolase enzymes. Org. Biomol. Chem. 8: 1301–1309. DOI: 10.1039/b924144b

2009

F. Baganz, S. Matosevic, L. Rios, G. Lye, A. Jadhav, N. Szita. 2009. Towards engineering of de novo pathways for the synthesis of chiral chemicals using in vitro and in vivo approaches. New Biotechnol. 25: 159-160. 10.1016/j.nbt.2009.06.511